Microsatellite DNA markers are consistently found to be more informative than other classes of markers in hexaploid wheat. The objective of this study was to develop new simple sequence repeat (SSR) markers from EST (Expressed sequence tag) sequences for wheat. The wheat EST sequences from GenBank/dbEST (from April 18, 2006 to February 4, 2007) were used to search for SSRs, and EST-SSR primer pairs were designed by using Primer5.0 software. The validity of primer pairs were detected using three wheat variet...