Purpose: We made this experiment after reading a lot of related reference books and documents about the influences of needling upon the changes of human body temperature. We took needling techniques as constant, acupuncture twist frequency as variable to make the experiment, made acupuncture on Sānyīnjiāo, ti chi of spleen channel with different twist frequency, and measured the temperature changes for Sānyīnjiāo, ti chi, and hypogastrium with thermal infrared imager. The resarch objective is to fin...