Zoysia matrella(L.) Merr.,namely manilagrass,is an excellent warm-season turfgrass species,which is widely used in golf courses,ground track fields and parks.As Zoysia matrella produces asexually by stolons in nature,conventional hybridization breeding procedure cannot be applied for its genetic improvenment.Thus modern biological technology such as somaclonal variation is an effective way to increase its resistance. In this experiment,embryogenic calluses induced from the stolons of manilagrass wer...