The technology of immobilization enzyme have been developing fast since 1960s. In the recent decades, research on usable immobilization carriers has been focused much attention in this field. The performance of immobilized enzyme depended on the properties and immobilized method of the enzyme carrier material. In this paper, the immobilization methods, properties and usage of immobilized enzyme were introduced, and then two kinds of carriers were designed and prepared. They were employed in immobili...