In accordance with the transgenic methods of Tremella fuciformis mediated by Agrobacterium EHA105 that previously established in our laboratory, our experiments used one hygromycin sensitive strain of Tremella fuciformis named Tr21-01 as a original strain. In our research, with the method of Freeze-thaw, the plasmid pBHg-BCA which consisted of the human insulin BCA gene as objective gene and hygromycin phosphotransferase gene as genetic markers transformed Tremella fuciformis mediated by Agrobacterium GV310...