Citrus was one of the most important fruit trees which was distributed in the tropics and subtropics, and it had an important role in economics. Conservation of germplasm resources took more and more attentions along with the development of technology. In this study, 67 accessions of citrus germplasm were collected for in vitro conservation which stemmed from mature nucellus embryos and citrus (Citrus reticulata‘Ponkan’) in vitro plantlets were used as the main test materials to optimize the preservative sy...