研究植物开花时间调控的分子机制对于调节植物繁殖和发育、提高作物产量和品质具有重大的意义。实验室前期研究从拟南芥T-DNA插入突变体库中筛选获得了一株早花突变体efs-1(early flowering in short mutant 1),通过Genome Walking技术和生物信息学方法鉴定出一个新的拟南芥开花调控相关基因EFS1,通过互补回复和超表达试验,确定了EFS1基因为拟南芥开花调控基因,利用RT-PCR和Real-Time PCR技术,初步确定了EFS1基因可能通过GA、春化和光周期途径调控拟南芥开花。为进一步明确EFS1基因在拟南芥开花调控途径中的功能,本试验利用反义RNA和RNAi技术,获得EFS1基因功能缺失突变体,验证了EFS1基因的功能;通过检测春化,赤霉素对花期和EFS1基因表达的影响,初步确定EFS1基因参与的拟南芥开花调控途径;通过构建及获得并鉴定开花关键基因的超表达、T-DNA插入突变体,为进一步明确EFS1基因所参与的拟南芥开花调控途径及在途径中的位置奠定基础。
1.春化和赤霉素处理拟南芥野生型、efs-1突变体、回复及超表达突变体,通过分析春化和赤霉素处理对花期及...
【英文摘要】
The flowering transformation process in higher plants is strictly controlled by both the inherent regulation and the exterior environmental signals. The flowering process is a significant one in the transition from vegetative growth to reproductive growth, which plays a crucial role in guarantying the successful reproduction and deciding the time of flowering. The investigation to the molecular mechanism regulated by flowering time in plants would make a significant contribution to regulating plant reproduc...