ZEN and DON are the toxic secondary metabolites mainly produced by Fusarium species, such as Fusarium culmorum, Fusarium graminearum and so on. ZEN and DON wich could induce reproduction toxicity, immunotoxicity, cytotoxicity and cancer are common contaminant in maize, barley, wheat and grain. Up to now, the currently used methods to detect ZEN, DON and other mycotoxins are instrument detection methods and immunoasssys. However the instrument detection methods require exorbitant expenses, time consu...