In this experiment, small vitrification cotyledonary somatic embryos ( SE ) after synchronization regulation from the LC2 embryogenic callus (EC) were used as the materials for the following studies in longan (Dimocarpus longan Lour. cv. Honghezi) :①difference of maturation of cotyledonary embryos (SE) growing on the maturation media with different concentrations of sucrose and polyethylene glycol (PEG);②two-dimensional gel electrophoresis analysis of proteins in cotyledonary embryos during normal m...