In this study, seeds, buds and inflorescence of Alocasia macrorrhiza were used as explants for studying various factors on in vitro culture, and an efficient in vitro regeneration system was established; the rhizome and inflorescence were used as explants for conducting callus induction and plant regeneration; plantlets in vitro were used as materials for finding out the suitable conditions of hydroponics in rooting, pH value and the concentration of nutrient solution.Then the physiological indexes ...