Organ regeneration in vitro is affected by many factors, but little is done to analyze systematically these factors. In this study, a one-step adventitious shoot regeneration method was developed using roots of Arabidopsis as explants. Compared with the traditional method, this method is efficient, simple and rapid. So it provides a good system to research the shoot regeneration in vitro. Basing on the system, we analyzed the effects of several factors, such as ecotype, explant, hormone type and horm...