The experiment were done in Guangxi Crop Genetic Improvement and Biotechnology Lab, Agricultural College of Guangxi University Vegetable Lab and Scientific Research base of Horticulture Research Institute of Guangxi Academy of Agricultural Sciences from 2006~2008. The diploid cucumis melo L. seeds were provided by Horticulture Research Institute of Guangxi Academy of Agricultural Sciences .Three concentrations of Colchicines, 0.2%, 0.4% and 0.6%, were used to shoot tips of seedling and seeds soaked f...