In this experiment,a high-efficiency and systematic transgenic procedure mediated by Agrobacterium tumefaciens with antisense ACS gene was developed from protocorm-like bodies(PLBs)in Dendrobium,and preliminary studies on in vitro flowering from plantlets without roots were also carried out.The main results were described as follows:
1 The plant regeneration system of Dendrobium was established.The studies on the efficiently transgenic establishment of the regeneration from stems,leaves and seed...