Works including the extraction and separation of flavonoids and polyasccharides from Aloe by microwave and aqueous two- phase systems have been reported in this paper. The optimal condition for microwave method is confirmed through orthogonal experiment about the extracting efficiency of flavonoids. The partitioning behaviors of flavonoids and Aloe polysaccharides in aqueous two-phase systems are investigated. The influences of composing of aqueous two-phase systems on the separation of flavonoids an...