The research actuality of interaction between small molecules with serum albumin in recent years has been reviewed and the interaction between some drugs with bovine serum albumin (BSA) was studied. Spectroscopy method has become firmly entrenched, particularly for the interaction of small molecules and macromolecules. However, the spectroscopy investigation combined with chemometrics in this field is limited. The application of chemometrics method- multivariate curve resolution-alternating least s...