银杏(Ginkgo biloba L. )属银杏科银杏属,为中生代第四纪冰川期的孓遗植物,有“活化石”之称。我国是银杏的原产地,许多地方有规模不等的银杏古树,湖北随州就是我国主要的银杏古树集中分布区之一。本研究采集随州121株古银杏的叶片样品和30株古银杏的种子样品为研究样本,利用RAPD和ISSR分子标记技术,从群体和个体2个层次,对随州古银杏的遗传多样性进行了分析研究。主要结果如下:
(1) 用9条RAPD引物和9条ISSR引物分别对随州大洪山区7个群体共121株古银杏的叶片DNA进行扩增,分别得到104条和107条清晰的扩增谱带,其中多态性带分别为103条和105条,多态性条带百分率(PPB)分别为99.04%和98.13%。RAPD所揭示的古银杏遗传多样性无论是物种水平(H_E=0.3241,H_O=0.4887)还是群体水平(H_E=0.2302,H_O=0.3440)均与ISSR分析所得的结果(物种水平:H_E=0.2516,H_O=0.3913;群体水平:H_E=0.1775,H_O=0.2697)相近。由RAPD所得的遗传一致度和由ISSR所得的遗传一致度之间的相...
【英文摘要】
Ginkgo biloba L., a living fossil belonging to Ginkgoaceae and Ginkgo, is one of the oldest remained tree species. China is the native land of Ginkgo biloba and there are lots of ancient Ginkgo trees in many places in the country. Suizhou of Hubei Province is one of the main concentrated distribution regions of ancient Ginkgo trees. In this paper, we collected leaf samples from 121 ancient Ginkgo trees and seed samples from 30 ancient Ginkgo trees in Suizhou as research samples, analyzed the genetic diversi...