Production of the laccase by Flammulina velutipes was investigated under flask culture. Different fermentative conditions were compared to investigate the influence on laccase secreted by Flammulina velutipes. Organic nitrogen is better than inorganic nitrogen for laccase secreted by Flammulina velutipes, and using peptone or yeastrel as nitrogen source in the culture after eight days fermentation the laccase activity arrived 45.7 IU/mL and 53.0 IU/mL respectively. Rich nitrogen is better too. Different car...