本文以褐牙鲆(Paralichthys olivacsus Temminck et Schlegel)为对象,分别以中国威海(W)、日本鸟取(J)和韩国丽水(K)野生群体及中国荣成养殖群体(C)为材料,采用RAPD和ISSR技术对野生群体(W)和养殖群体(C)的遗传多样性研究进行了比较分析;利用ISSR技术对W、J和K三个野生群体的遗传结构和遗传多样性进行了研究;用ISSR技术对育种群体中单对交配后代的大型个体和小型个体进行对比分析。主要实验结果如下:
1:利用RAPD和ISSR两种分子标记技术,分析了山东威海近海野生和养殖褐牙鲆的遗传多样性差异。用10个RAPD引物对两个样本各30个个体的基因组DNA进行扩增,共获得110个重复性好且带谱清晰的扩增位点,片段大小在200～2500bp之间,野生样本和养殖样本的多态位点比例分别为59.09%和60.90%、Shannon多样性值分别为16.563和16.917,野生样本内平均遗传距离为0.17427,养殖样本为0.17553,样本间平均遗传距离为0.17419。用6个ISSR引物共在两样本中检测到161个位点,野生样本有111个(6...
【英文摘要】
Two type molecular markers, randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to detect the genetic diversity between wild and cultivated populations of Olive flounder. ISSR was also used to detect the genetic structures of 3 wild populations and differences between large and small groups in the offspring of pair mating experiment. The results were as follows.1: RAPD and ISSR analyses were used to detect the genetic diversities in wild and cultured populations of t...
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