This paper was summarized the investigative status of the application of laser ablation of biotissue in bio-medical domain. At this time, more and more scientists, who are medicals, biologists, physicists, etc, pay attention to the laser technologies in the study of diagnosing and therapy diseases. This thesis is devoted to study relationship between tissue ablation threshold and dose by combining animal experimental means and numerical analysis.At first, a short introduction to the history of the study of ...