本文利用PCR-RFLPs(Restriction fragment length polymorphism,RFLPs)方法,对130头苏淮母猪的IGF1、MSTN、MyoG、RYR1、RBF4、PRLR、ESR等7个候选基因进行了多态性分析,并检测了侯选基因各基因型之间在生长、繁殖性状上的差异,旨在通过这些分子遗传标记加快苏淮猪的培育进展,为苏淮猪的进一步培育提供一定的理论依据。
研究结果表明,苏淮猪7个候选基因均表现出多态性。在IGF1、MyoG、RBP4、PRLR、ESR等5个基因座位上均检测到2个等位基因,表现为三种基因型;在MSTN、RYR1等基因座位上均检测到2个等位基因,RYR1表现出2种基因型,MSTN表现为一种杂合子基因型。PCR-RFLPs检测到苏淮猪氟烷基因n的基因频率为0.1318。
哈代-温伯格平衡定律检测结果表明:苏淮猪7个功能基因中,ESR、MSTN和MyoG处于遗传不平衡状态。其余4个基因IGF1、RYR1、PRLR和RBP4处于遗传平衡状态。
苏淮猪7个侯选基因各基因型间生长、繁殖性能的差异...
【英文摘要】
The morphisms of seven functional genes ( IGF1, MSTN, MyoG, RYR1, RBP4, PRLR and ESR) and their relationship to reproduction performance were investigated by PCR-RFLPs in this paper, to provide the theoretical basis for further selection of Suhuai pigs using these molecular genetic markers.The results showed that the seven genes all had polymorphisms. IGF1, MyoG, RBP4, PRLR and ESR genes were found having 2 alleles and 3 genotypes by PCR-RFLPs, 2 allels and 2 genotypes were detected in RYR1 gene, 2 allels a...
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