Peony is a traditional flower with great variety and wild resource. But the present preservation method is single and the lost of resource seriously. In this paper, cryopreservation procedure and physiological and physiochemical changes of peony pollen after and during LN2 storage were studied. Cryopreservation procedure was established, and the mechanism of cryopreservation was investigated. The results show:1. Cryopreservation can maintain peony pollen viability for a long time. Germination rate can stil...