Three kinds of virus-free plants about Chrysanthemum, Tulip and Caryophyllus were studied in the paper. In test of virus-free Chrysanthemum plant, the Chrysanthemum stem tips with a length of 0.5-0.8mm is suitable for explants, which came from Guoqing Chrysanthemum with CMV. They were inoculated in MS medium with different consistency of BA and NAA. The results showed that the proportion of BA and NAA affected callus inducing and growing. Callus grew well on MS+BA2.0mg/l+NAA0.1mg/l, 96.0% explants formed. C...