In the present research papain and trypsin were used to hydrolyze spleen protein and then the antioxidant properties of the hydrolysis were analyzed. It was done to abtain the optimal condition of hydrolysis of spleen protein. The optimum hydrolysis parameters were: the enzyme application accounted for 5.0%(enzyme/substrate),reaction conditions of pH 7.0, temperature 60#, time 5.5 hours and the degree of hydrolysis being 60.59% with stronger antioxidative activities. The hydrolysate was fractionated by gel ...