The callus induction and subculture of Eucommia ulmoides Oliv. are studied from the aspects of basic media, installing method, type of explant, sampling time, culturing conditions, phytohormones etc. The influence of the condition of tissue culture on the contents of metabolites contained in the callus is also studied preliminarily. The results are as follows:
1. Among the 8 media tested, MS is suitable for the callus induction of immature shoots of E. ulmoides Oliv. 65 medium howeve...