Objectives: To explicate the composition of microflora in active sludge of sewage treatment. To obtain proper strains fitting for genes manipulating to enhance the ability of denitrification. To clone napEFDABC gene cluster from Pseudomonas strain G179 and express it in the strains we had screened.
Methods and Results: First of all, we counted the population of bacteria in the active sludge. Using their biochemical and growth properties identify those strains we had isolated. We not...