The BABL/C mice were immunized with the compound of Trx-LHRH and trx antibody. When the titre came to 1:104, the spleen cells were fused with the SP2/0 by PEG-1500. The hybridoma surprunt was detected with indirect ELISA and inhibitory ELISA. The hybridoma strains D4B7 and B6E6 secreting Mcab against LHRH were obtained by dilluting several times and the ascite was identified with Westen-blot. A double antibody sandwich ELISA was established to detect the quantity of effective content of the emas...