A high quality cDNA library of M. Red stark ing fruit has been constructed. The total RNA of apple fruits has been extracted by using CTAB method. The single strand cDNA was synthesized from mRNA with MMLV reverse transcriptase, then double strand cDNA was synthesized by using PCR. Double strand cDNA size fractionation was completed by CHROMA SPIN-400 column, so the low molecular weight cDNA fragments shorter than 500 bp could be removed out. cDNA was packaged using the A. phage packaging system...