To develop a method of germinating and sterilizing B.Subtilis spores with minimal heating was studied by the combined effect of oscillatory and pulsed UHP, pressurization times, and operations cycles. The condition above room temperature was benefit to UHP sterilization. At 40℃,seven pressurization cycles with the first pressure at 200MPa for 5min,followed by an ambient pressure pause for 1min, then another 700MPa pressurization for 5min, decreased the spores count from 107 to 102-103cells/ml. T...