The use of insecticides does not only bring benefit to human beings but also cause serious environmental pollution. Many insects including Culex pipiens can product detoxificase that can catalyze and degrade many xenobiotic agents besides pesticides, so it becomes harder to kill them. But the use of insect detoxificase provides a novel method in bioremediation of pesticides contamination ,so this thesis is aimed at fusion expressing the detoxificase(esterase B1) at high level. It will pave the w...