In this paper, Several proteolytic enzymes (such as Alcalase, Neutrase, Flavourzyme, A.S.1398) were studied systematically and individually, the best making condition of the four enzymes were confirmed. The results showed that under the optimum conditions the sequence of each enzyme's hydrolysis capacity was: Alcalase>Flavourzyme>Neutrase>A.S.1398,and the optimum condition of Alcalase was confirmed that the temperature =58?, substrate concentration =5%, enzyme concentration =10%, hydrolysis time...