System of cucumber haploid induction from unpollinated ovaries has been successfully established in Tianjin Cucumber Research Institute. The frequency of the regeneration has been over 20%. But the ploidy of plant population obtained are always a distribution of haploid, diploid, triploid, tetraploid and other ploidy levels. Ploidy determination was very important for the utilization of the plants. Haploid plants cannot be used in breeding and further genetic study until they were doubled.
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