In this paper,the protoplast preparation conditions of Agrocybe cylindracea strain were studied,these conditions include different media,different temperatures,different pH,different reaction time,different mycelial age and different osmotic stabilizers.The results showed that:after the mycelium cultured for 5 days in liquid oscillation shaking,with 0.6 mol/L mannitol or MgSO4 as osmotic stabilizer,and a concentration of 2% of the fungal wall dissolving enzyme(lywallzyme) in the pH6.0,temperature 31 ℃ for 2...