The experiment was used ultrasonic wave assisted method for the extraction of the total flavonoids from Ardisia pusilla.The extraction of the total flavonoids from Ardisia pusilla were carried on by single factor experiment and orthogonal experiment.The results showed that optimum condition for the extraction was as follows:solid-to-liquid ratio was 1∶40,ultrasonic power was 300 W,ultrasonic time was 10 min and extraction temperature was 80℃,and extraction rate was 5.68%.