[Objective]Five methods were used to determine the pollen vitality of different lines rape to find out their best determination method.[Method]Five determination methods were sucrose-based germination method,acetocarmine dyeing method,TTC dyeing method,I2-KI dyeing method and red ink dyeing method,respectively.[Result]The results showed that the pollen vitality values of different lines rape determined by red ink dyeing method and TTC dyeing method were on the high side,while that was on the low side determ...