Pathogen causing anthracnose on Cymbidium ensifolium in Zhangzhou and Fuzhou,Fujian which had was isolated and identified and the culture condition was optimized.The result showed that the pathogen of anthracnose on Cymbidium ensifolium was Colletotrichum gloeosporioides.The optimal culture condition was: PDA medium,28 ℃,pH7.0,complete darkness or alternation of light and darkness every 12 hours,sucrose or starch for carbon source and NH4NO3 for nitrogen source.