HPLC method was developed to determine the contents of luteolin and luteoloside in different parts of Lonicera japonica(flower,leaf,epidermal stem and stem).Samples were determined by HPLC on Aglient Zorbax SB-C18column.Luteolin was isolated using methanol(φ=0.02%)-H3PO4(φ=50%) as the mobile phase,the mobile phase of isolating luteoloside was 0.5% acetonitrile(A) and-acetic acid(B),gradient elution V(A)∶V(B) from 1∶9 to 3∶7.The flow rate was 0.3 mL/min,column temperature was 30 ℃,UV detection wavelength was...