Conidiation-deficiency transformant BCG-183 was obstained from more than 400 ATMT transformants.The genomic DNA of wild type and BCG-183 were extracted by CTAB method,and southern blot showed that one copy of T-DNA had inserted into the mutant's DNA.By analysing the phenotype of the mutant,we found that mutant's hyphae were more dense than the wild type,while its colony was white.The mutant didn't produce conidium.And the cell-wall-degrading enzyme activities were stronger than that of the wild type′s.T-DNA...
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