Poly(D,L-lactide-co-glycolide)(PLGA) has attracted much attention due to its good biodegradable and biocompatible properties.PLGA has been shown great clinical application of controlled drug delivery systems.Focusing on the low drug loading and encapsulation efficiency of PLGA microspheres,this article reviews the variables influencing the drug loading and encapsulation efficiency,which may have significant implications for the research on the drug loading and encapsulation efficiency.