以茂名野生动物园斑鼻羚体内分离出的毛首线虫为研究对象,用保守引物PCR扩增其核糖体DNA(rDNA)的内转录间隔区(ITS)和5.8 S序列,并进行克隆、转化、测序和序列分析,对样品进行分子鉴定。结果获得2个毛首线虫样品的ITS及5.8 S rDNA序列,总长为1 316 bp,样品间序列相似性为99.2%。将序列与GenBankTM公布的相关序列进行比较分析,结果显示与羊毛首线虫的ITS1、5.8 S和ITS2序列相似性高,分别为97.3%~97.6%、100%和97.8%~98.0%,表明斑鼻羚体内分离的毛首线虫属于羊毛首线虫。
【英文摘要】
The aims of this study were to amplify and analyze the sequences of internal transcribed spacers(ITS) of ribosomal DNA(rDNA) of Trichuris samples isolated from a Addax nasomaculatus in Maoming Safari Park,China.The DNA of two samples,namely MM3 and MM4,were extracted and the ITS sequences were amplified by PCR with primers NC5 and NC2.The amplicons were cloned into pGEM-T Easy vector and the inserts were successfully sequenced.The results revealed that the total length of ITS and 5.8 S rDNA of the two Trich...