The high performance liquid chromatography(HPLC) method was established for determining the swine plasma concentration of lincomycin and gentamicin compound injection.The plasma samples were extracted by acetonitrile and trichloroacetic acid,purified by C18 Column of solid-phase extractio(SPE),concentrated by Nitrogen,then detemined by diode array detector.The result showed that the linear response in the range of 0.5-50 μg·mL-1 for lincomycin hydrochloride and in the range of 0.33-33 μg·mL-1 for gentamicin...