The objective of this study was to extract and purify total flavonoids from Cynomorium songaricum.AB-8 macroporous resin was used in the experiment.The result showed that the purity of total flavonoids was 97.278%;the adsorption ratio and elution ratio were 81.933 mg/g and 53.164 mg/g respectively.The study indicates that the AB-8 type of macroporous adsorption resin has high adsorption and elution ratio for separation and purification of the total flavonoids from C.songaricum.