The preparation technology of theaflavins with dynamic two-phase system using tea polyphenols as raw material was introduced.The reaction conditions were optimized with blank column orthogonal experiment design by SPSS.The reaction factors such as polyphenol oxidase(PPO)concentration,the ratio of water/ethyl acetate and reaction time were studied.It was indicated that the optimum reaction conditions were as follows.PPO concentration was 1600U/g,the ratio of water/ethyl acetate was 1:0.5,reaction time was fo...