Several main factors influencing inter-simple sequence repeat(ISSR) reaction were analyzed in order to establish ISSR molecular marker system in Zizyphus jujube genus.The optimal system was in a reaction volume of 25 μL including: 2.5 ?L 10×buffer,50 ng template DNA,2.5 mmol·L-1 Mg2+,0.3 mmol·L-1 dNTPs,0.3 μmol·L-1 primer,2 U TaqDNA polymerase.The optimal amplification program was with the following cycling parameters: 94°C for 2 min;35 cycles of 94°C for 1 min;specified annealing temperature for 1 min,72°C...
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