Different cell-wall breaking methods were taken for extracting glutamate decarboxylase(GAD) from Lactobacillus brevis.The effects of cell wall breaking methods on activity of GAD were studied.The results showed that the ultrasonic method was beneficial to the release of GAD from the cells.The results of orthogonal test showed that the best process for cell-wall breaking with ultrasonic was: ultrasonic power of 220 W,working time of 20 min,the ratio of work time/intermission time of 5 s/10 s.
【基金】
天津市高等学校科技发展基金计划项目(No.20090601)
【更新日期】
2012-01-06
【分类号】
S816.7
【正文快照】
短乳杆菌(Lactobacillus brevis TCCC13007)属于GRAS(generally recognized as safe)级乳酸菌,其细胞内具有较高活性的谷氨酸脱羧酶(glutamate decarboxylase,GAD,EC4.1.1.15),该酶可催化底物谷氨酸或谷氨酸钠盐进行脱羧反应,生成γ-氨基丁酸和二氧化碳。γ-氨基丁酸是一种具有
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