[Objective] The aim was to study the high performance liquid chromatography(HPLC) determination of endogenous hormones in agavybrid No.11648 leaves.[Method] Combining the hormone extraction methods of enzyme linked immunoassay,Gibberellin acid(GA3),3-indoleacetic acid(IAA),abscisic acid(ABA) and zeatin(ZT) in mature leaves of agavybrid No.11648 were determined by HPLC.[Result] The procedure could analyze the GA3,IAA,ABA and ZT in combination,and their recoveries were 78.81%,104.96%,81.24% and 100.55% respec...