[Object] The paper was to study the effect of cryopreservation in liquid nitrogen on germinability and vigor of wheat seeds,in order to provide the theoretical basis for the cryopreservation of wheat germplasm resources.[Method]The seeds of four wheat varieties including Zhengmai 9023,Wanmai 48,Yangmai 158 and Annong 1032(waxy wheat)naturally dried after harvest were selected and sealed in plastic frozen pipes,then cryopreservated in liquid nitrogen for 7 d.Subsequently,the seeds were taken out and thawed s...