With the transgenic maize as experiment material,the PCR system for detecting exogenous gene was optimized in extracted DNA quality,Mg2+ concentration,annealing temperature and primer dosage.In the optimized system,the dosage of 25 mmol/L Mg2+ was reduced from 1.5 μl to 1.2 μl;the dosage of 12.5 mmol/L primer was reduced from 1 μl to 0.5 μl;the annealing temperature was increased by 2℃.The Touchdown PCR reaction conditions were 95℃ for 5 min;95℃ for 1 min,62.5℃ for 1 min(1℃ decreased after each cycle),72℃ f...
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