Using the orthogonal design to optimize ISSR-PCR amplification system on jujube in four levels of five factors(Taq DNA polymerase,Mg2+,DNA template,dNTP,and primer)respectively.PCR result showed that each factor in different levels has significant effect on the result of PCR,the most remarkable factor was the quantity of Taq DNA polymerase.The optimum level of ISSR-PCR system for jujube was established.The total reaction system was 25 μL and contained 1.0U Taq DNA polymerase,1.0 mmol/L Mg2+,1.0 μL DNA templ...
枣Z iz iphus jujuba M ill,为鼠李科Rham naceae枣属Z iz iphusM ill植物,是原产我国的特产果树[1]。枣树已有7000多年的栽培利用历史,资源丰富,具有重要的经济和生态价值。利用分子标记技术进行枣树种质资源鉴定和分类、构建遗传连锁图谱和基因定位等[2]已取得一定进展,但多?
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