[Objective] To observe the protective effect of swertiamarin on H2O2-induced apoptosis of mice cardiomyocytes and explore its protective mechanism.[Methods] Cultured with selective plating method,the cell viability,SOD and MDA contents of SD mice cardiomyocyte were determined;the expression rates of apoptosis proteins bcl-2 and Bax were detected with immunocytochemical technique,and the cell apoptosis was detected by acridine orange fluorescent staining.[Results] The viability and SOD activity of cardiomyoc...
【英文摘要】
[Objective] To observe the protective effect of swertiamarin on H2O2-induced apoptosis of mice cardiomyocytes and explore its protective mechanism.[Methods] Cultured with selective plating method,the cell viability,SOD and MDA contents of SD mice cardiomyocyte were determined;the expression rates of apoptosis proteins bcl-2 and Bax were detected with immunocytochemical technique,and the cell apoptosis was detected by acridine orange fluorescent staining.[Results] The viability and SOD activity of cardiomyoc...