Objective To select the best technique and parameter of the separation and purification of the total flavonoids from Baihuasheshecao ( Herba Hedyotis Diffusa) with macroporous resin. Method The types of macroporous resins,concentration of sample solution,pH values,volume of sample,and concentration, quantity and flow velocity of eluant were reviewed by using the single factor investigation taking the content and recovery of the total flavonoids from Baihuasheshecao as the investigative indexes. Result The p...